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Human CCDC47 (Coiled-Coil Domain-containing Protein 47) ELISA Kit
Human CCDC47 (Coiled-Coil Domain-containing Protein 47) ELISA Kit
The Human (CCDC47) Coiled-Coil Domain-containing Protein 47 ELISA Kit measures Coiled-Coil Domain-Containing Protein 47 in samples. The plate has been pre-coated with Human CCDC47 antibody. CCDC47 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CCDC47 Antibody is added and binds to CCDC47 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCDC47 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CCDC47. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5065Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.22 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Component of the PAT complex, an endoplasmic reticulum (ER)-resident membrane multiprotein complex that facilitates multi-pass membrane proteins insertion into membranes (PubMed: 32814900). The PAT complex acts as an intramembrane chaperone by directly interacting with nascent transmembrane domains (TMDs), releasing its substrates upon correct folding, and is needed for optimal biogenesis of multi-pass membrane proteins (PubMed: 32814900). WDR83OS/Asterix is the substrate-interacting subunit of the PAT complex, whereas CCDC47 is required to maintain the stability of WDR83OS/Asterix (PubMed: 32814900). The PAT complex favors the binding to TMDs with exposed hydrophilic amino acids within the lipid bilayer and provides a membrane-embedded partially hydrophilic environment in which the first transmembrane domain binds (PubMed: 32814900). Component of a ribosome-associated ER translocon complex involved in multi-pass membrane protein transport into the ER membrane and biogenesis (PubMed: 32820719). Involved in the regulation of calcium ion homeostasis in the ER (PubMed: 30401460). Required for proper protein degradation via the ERAD (ER-associated degradation) pathway (PubMed: 25009997). Has an essential role in the maintenance of ER organization during embryogenesis (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Coiled-Coil Domain-Containing Protein 47
Regulatory Status
For Research Use Only
