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Human CD177 (Cd177 Antigen) ELISA Kit
Human CD177 (Cd177 Antigen) ELISA Kit
The Human (CD177) Cd177 Antigen ELISA Kit measures CD177 antigen in samples. The plate has been pre-coated with Human CD177 antibody. CD177 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD177 Antibody is added and binds to CD177 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD177 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD177. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5016Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.26 ng/mL
Detection Range
0.5-100 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, CD & Adhesion molecule
Background
In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
CD177 antigen
Regulatory Status
For Research Use Only
