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Human CD244 (Natural Killer Cell Receptor 2B4) ELISA Kit
Human CD244 (Natural Killer Cell Receptor 2B4) ELISA Kit
The Human (CD244) Natural Killer Cell Receptor 2B4 ELISA Kit measures Natural Killer Cell Receptor 2B4 in samples. The plate has been pre-coated with Human CD244 antibody. CD244 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD244 Antibody is added and binds to CD244 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD244 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD244. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6860Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.13 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, CD & Adhesion molecule
Background
Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed: 10359122, PubMed: 8376943, PubMed: 11714776). Activating function implicates association with SH2D1A and FYN (PubMed: 15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed: 10934222, PubMed: 15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed: 8376943, PubMed: 11714776). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed: 10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed: 11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD488 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Natural Killer Cell Receptor 2B4
Regulatory Status
For Research Use Only
