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Human CD3 (Cluster of Differentiation 3) ELISA Kit

Human CD3 (Cluster of Differentiation 3) ELISA Kit

The Human (CD3) Cluster of Differentiation 3 ELISA Kit measures Cluster of Differentiation 3 in samples. The plate has been pre-coated with Human CD3 antibody. CD3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD3 Antibody is added and binds to CD3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0221Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.01 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas CD & Adhesion molecule
Background CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Cluster of Differentiation 3
Regulatory Status For Research Use Only
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