The Human (CD79A) B-cell Antigen Receptor Complex-associated Protein Alpha Chain ELISA Kit measures B-cell antigen receptor complex-associated protein Alpha chain in samples. The plate has been pre-coated with Human CD79A antibody. CD79A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD79A Antibody is added and binds to CD79A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD79A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD79A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundRequired in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteB-cell antigen receptor complex-associated protein Alpha chain
