The Human (CELA3B) Chymotrypsin-like Elastase Family Member 3B ELISA Kit measures Chymotrypsin-like elastase family member 3B in samples. The plate has been pre-coated with Human CELA3B antibody. CELA3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CELA3B Antibody is added and binds to CELA3B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CELA3B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CELA3B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundChymotrypsin-like elastase family member 3B also known as elastase-3B, protease E, or fecal elastase is an enzyme that in humans is encoded by the CELA3B gene. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteChymotrypsin-like elastase family member 3B
