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Human Cell Surface Glycoprotein Muc18 (MCAM) ELISA Kit
Human Cell Surface Glycoprotein Muc18 (MCAM) ELISA Kit
This ELISA kit is designed to detect Human Cell Surface Glycoprotein Muc18 (Human MCAM). The assay plate has been pre-coated with mouse anti-Human CD146 monoclonal antibody. When the sample containing CD146 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human CD146 Antibody is added to the wells and binds to CD146 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human CD146 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE023
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
2.68 pg/mL
Detection Range
11-700 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cancer, Cardiovascular, Immunology, Signal Transduction, Stem cells
Background
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Cell surface glycoprotein MUC18
Regulatory Status
For Research Use Only
