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Human CFI (Complement Factor I) ELISA Kit
Human CFI (Complement Factor I) ELISA Kit
The Human (CFI) Complement Factor I ELISA Kit measures Complement Factor I in samples. The plate has been pre-coated with Human CFI antibody. CFI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CFI Antibody is added and binds to CFI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CFI antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CFI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3502Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
1.42 ng/mL
Detection Range
3-900 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology
Background
Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three Peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed: 7360115, PubMed: 17320177). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed: 2141838, PubMed: 9605165, PubMed: 12055245). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed: 28671664). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Complement Factor I
Regulatory Status
For Research Use Only
