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Human CLEC4D (C-type Lectin Domain Family 4 Member D) ELISA Kit
Human CLEC4D (C-type Lectin Domain Family 4 Member D) ELISA Kit
The Human (CLEC4D) C-type Lectin Domain Family 4 Member D ELISA Kit measures C-type lectin domain family 4 member D in samples. The plate has been pre-coated with Human CLEC4D antibody. CLEC4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CLEC4D Antibody is added and binds to CLEC4D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CLEC4D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CLEC4D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5133Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.028 ug/mL
Detection Range
0.05-20 ug/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology
Background
Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of pathogen-associated molecular patterns (PAMPs) of bacteria and fungi (PubMed:23602766, PubMed:23911656).
The PAMPs include alpha-mannans on C.albicans hypheas and mycobacterial trehalose 6,6'-dimycolate (TDM) (PubMed:23602766, PubMed:23911656).
Interacts with signaling adapter Fc receptor gamma chain/FCER1G, likely via CLEC4E, to form a functional complex in myeloid cells (By similarity).
Binding of mycobacterial TDM or C.albicans alpha-mannans to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (PubMed:23602766, PubMed:23911656).
The heterodimer formed with CLEC6A is active against fungal infection (PubMed:23911656).
Functions as an endocytic receptor (PubMed:14971047).
May be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T-cells (PubMed:14971047). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
C-type lectin domain family 4 member D
Regulatory Status
For Research Use Only
