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Human Coagulation Factor IX (F9) ELISA Kit

Human Coagulation Factor IX (F9) ELISA Kit

This ELISA kit is designed to detect Human Coagulation Factor IX (Human F9). The assay plate has been pre-coated with mouse anti-Human Factor IX monoclonal antibody. When the sample containing Factor IX is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Factor IX Antibody is added to the wells and binds to Factor IX in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Factor IX present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE198
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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Product Details

Species Reactivity Human
Sensitivity 44.93 pg/mL
Detection Range 140.63-9000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cardiovascular, Immunology
Background Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Coagulation factor IX
Regulatory Status For Research Use Only
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