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Human CRISP1 (Cysteine-rich Secretory Protein 1) ELISA Kit
Human CRISP1 (Cysteine-rich Secretory Protein 1) ELISA Kit
The Human (CRISP1) Cysteine-rich Secretory Protein 1 ELISA Kit measures Cysteine-rich secretory protein 1 in samples. The plate has been pre-coated with Human CRISP1 antibody. CRISP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CRISP1 Antibody is added and binds to CRISP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CRISP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CRISP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5147Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.38 ng/mL
Detection Range
10-2000 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Cysteine-rich secretory protein 1 is a cysteine-rich secretory protein that in humans is encoded by the CRISP1 gene. Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cysteine-rich secretory protein 1
Regulatory Status
For Research Use Only
