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Human CRK (Adapter Molecule Crk) ELISA Kit

Human CRK (Adapter Molecule Crk) ELISA Kit

The Human (CRK) Adapter Molecule Crk ELISA Kit measures Adapter Molecule Crk in samples. The plate has been pre-coated with Human CRK antibody. CRK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CRK Antibody is added and binds to CRK in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CRK antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CRK. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4066hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.021 ng/mL
Detection Range 0.05-15 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. [Isoform Crk-II]: Regulates cell adhesion, spreading and migration (PubMed: 31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed: 19004829). May regulate the EFNA5-EPHA3 signaling (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Adapter Molecule Crk
Regulatory Status For Research Use Only
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