1
/
of
1
Human CTNNB1 (Catenin Beta 1) ELISA Kit
Human CTNNB1 (Catenin Beta 1) ELISA Kit
The Human (CTNNB1) Catenin Beta 1 ELISA Kit measures Catenin Beta 1 in samples. The plate has been pre-coated with Human CTNNB1 antibody. CTNNB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CTNNB1 Antibody is added and binds to CTNNB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CTNNB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CTNNB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2396Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
5.08 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Key downstream component of the canonical Wnt signaling pathway (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed: 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed: 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed: 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed: 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Catenin Beta 1
Regulatory Status
For Research Use Only
