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Human CYP1A1 (Cytochrome P450 1A1) ELISA Kit
Human CYP1A1 (Cytochrome P450 1A1) ELISA Kit
The Human (CYP1A1) Cytochrome P450 1A1 ELISA Kit measures Cytochrome P450 1A1 in samples. The plate has been pre-coated with Human CYP1A1 antibody. CYP1A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP1A1 Antibody is added and binds to CYP1A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP1A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP1A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3222Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.25 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed: 15041462, PubMed: 18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed: 15041462, PubMed: 19965576, PubMed: 20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed: 20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed: 15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed: 21068195). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytochrome P450 1A1
Regulatory Status
For Research Use Only
