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Human CYP24A1 (1, 25-Hydroxyvitamin D,1, 25-(OH)D) ELISA Kit
Human CYP24A1 (1, 25-Hydroxyvitamin D,1, 25-(OH)D) ELISA Kit
The Human (CYP24A1) 1, 25-Hydroxyvitamin D,1, 25-(OH)D ELISA Kit measures 1,25-Hydroxyvitamin D in samples.
Catalog No:
E1919Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
3.14 pmol/L
Detection Range
7-1500 pmol/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer
Background
A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed: 24893882, PubMed: 15574355, PubMed: 8679605, PubMed: 11012668, PubMed: 16617161, PubMed: 29461981). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed: 24893882, PubMed: 15574355). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed: 11012668, PubMed: 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed: 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed: 8679605). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
1,25-Hydroxyvitamin D
Regulatory Status
For Research Use Only
