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Human CYP27A1 (Sterol 26-hydroxylase, Mitochondrial) ELISA Kit
Human CYP27A1 (Sterol 26-hydroxylase, Mitochondrial) ELISA Kit
The Human (CYP27A1) Sterol 26-hydroxylase, Mitochondrial ELISA Kit measures Sterol 26-Hydroxylase,Mitochondrial in samples. The plate has been pre-coated with Human CYP27A1 antibody. CYP27A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP27A1 Antibody is added and binds to CYP27A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP27A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP27A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3869Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.89 ng/L
Detection Range
10-4000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (PubMed: 9660774, PubMed: 12077124, PubMed: 21411718, PubMed: 28190002). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways (PubMed: 9660774, PubMed: 1708392, PubMed: 11412116, PubMed: 2019602, PubMed: 7915755, PubMed: 9186905, PubMed: 9790667). May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (PubMed: 9660774, PubMed: 12077124). Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation (PubMed: 28190002). Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium (PubMed: 21411718). May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PubMed: 15465040). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Sterol 26-Hydroxylase,Mitochondrial
Regulatory Status
For Research Use Only
