The Human (CYP27B1) 25-Hydroxyvitamin D-1 Alpha Hydroxylase, Mitochondrial ELISA Kit measures 25-Hydroxyvitamin D-1 Alpha Hydroxylase,Mitochondrial in samples. The plate has been pre-coated with Human CYP27B1 antibody. CYP27B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP27B1 Antibody is added and binds to CYP27B1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP27B1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP27B1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundA cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed: 10518789, PubMed: 9486994, PubMed: 22862690, PubMed: 10566658, PubMed: 12050193). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed: 10518789, PubMed: 22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed: 10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed: 22862690). Source: UniProt Consortium (2025)
