1
/
of
1
Human CYP2B6 (Cytochrome P450 2B6) ELISA Kit
Human CYP2B6 (Cytochrome P450 2B6) ELISA Kit
The Human (CYP2B6) Cytochrome P450 2B6 ELISA Kit measures Cytochrome P450 2B6 in samples. The plate has been pre-coated with Human CYP2B6 antibody. CYP2B6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP2B6 Antibody is added and binds to CYP2B6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP2B6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP2B6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3083Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
9.77 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed: 21289075, PubMed: 12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed: 21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed: 21289075, PubMed: 12865317). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed: 11695850, PubMed: 22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed: 11695850). Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytochrome P450 2B6
Regulatory Status
For Research Use Only
