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Human CYP2R1 (Vitamin D 25-hydroxylase) ELISA Kit
Human CYP2R1 (Vitamin D 25-hydroxylase) ELISA Kit
The Human (CYP2R1) Vitamin D 25-hydroxylase ELISA Kit measures Vitamin D 25-Hydroxylase in samples. The plate has been pre-coated with Human CYP2R1 antibody. CYP2R1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP2R1 Antibody is added and binds to CYP2R1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP2R1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP2R1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3860Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.19 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular, Nutrition
Background
A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol) (PubMed: 12867411, PubMed: 15465040, PubMed: 18511070). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives (PubMed: 15465040, PubMed: 18511070). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed: 12867411, PubMed: 15465040, PubMed: 18511070). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Vitamin D 25-Hydroxylase
Regulatory Status
For Research Use Only
