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Human CYP3A4 (Cytochrome P450 3A4) ELISA Kit
Human CYP3A4 (Cytochrome P450 3A4) ELISA Kit
The Human (CYP3A4) Cytochrome P450 3A4 ELISA Kit measures Cytochrome P450 3A4 in samples. The plate has been pre-coated with Human CYP3A4 antibody. CYP3A4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP3A4 Antibody is added and binds to CYP3A4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP3A4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP3A4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3081Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
6.68 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism
Background
A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids (PubMed: 10681376, PubMed: 11093772, PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15373842, PubMed: 15764715, PubMed: 20702771, PubMed: 19965576, PubMed: 21490593, PubMed: 21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 2732228, PubMed: 14559847, PubMed: 12865317, PubMed: 15373842, PubMed: 15764715, PubMed: 21576599, PubMed: 21490593). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317). Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone (PubMed: 2732228, PubMed: 15373842, PubMed: 15764715, PubMed: 22773874). Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones (PubMed: 2732228, PubMed: 15373842, PubMed: 15764715). Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis (PubMed: 21576599). Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA) (PubMed: 9435160). Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond (PubMed: 19965576). Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed: 20702771). Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed: 10681376). Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed: 11093772). Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol) (PubMed: 11159812). Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole (PubMed: 10759686). Hydroxylates antimalarial drug quinine (PubMed: 8968357). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed: 11695850). Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed: 29461981). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytochrome P450 3A4
Regulatory Status
For Research Use Only
