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Human CYP3A5 (Cytochrome P450 3A5) ELISA Kit
Human CYP3A5 (Cytochrome P450 3A5) ELISA Kit
The Human (CYP3A5) Cytochrome P450 3A5 ELISA Kit measures Cytochrome P450 3A5 in samples. The plate has been pre-coated with Human CYP3A5 antibody. CYP3A5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP3A5 Antibody is added and binds to CYP3A5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP3A5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP3A5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3570Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.14 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins (PubMed: 2732228, PubMed: 10681376, PubMed: 11093772, PubMed: 12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 12865317, PubMed: 2732228, PubMed: 10681376, PubMed: 11093772). Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed: 12865317). Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione (PubMed: 2732228). Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed: 10681376). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed: 11093772). Also involved in the oxidative metabolism of xenobiotics, including calcium Channel blocking drug nifedipine and immunosuppressive drug cyclosporine (PubMed: 2732228). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytochrome P450 3A5
Regulatory Status
For Research Use Only
