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Human DDB2 (DNA Damage-binding Protein 2) ELISA Kit
Human DDB2 (DNA Damage-binding Protein 2) ELISA Kit
The Human (DDB2) DNA Damage-binding Protein 2 ELISA Kit measures Dna Damage-Binding Protein 2 in samples. The plate has been pre-coated with Human DDB2 antibody. DDB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DDB2 Antibody is added and binds to DDB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DDB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DDB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5189Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.59 ng/L
Detection Range
20-3800 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed: 10882109, PubMed: 11278856, PubMed: 11705987, PubMed: 9892649, PubMed: 12732143, PubMed: 15882621, PubMed: 16473935, PubMed: 18593899). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed: 10882109, PubMed: 11278856, PubMed: 11705987, PubMed: 16260596, PubMed: 12944386, PubMed: 1$475.00|$350.001237). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed: 10882109, PubMed: 11278856, PubMed: 11705987, PubMed: 16260596, PubMed: 12944386). Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1) (PubMed: 12732143, PubMed: 15882621, PubMed: 16473935, PubMed: 18593899, PubMed: 26572825). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed: 16678110, PubMed: 16473935). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed: 16678110, PubMed: 16473935). The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed: 15882621). The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR (PubMed: 26572825). [Isoform D1]: Inhibits UV-damaged DNA repair. [Isoform D2]: Inhibits UV-damaged DNA repair. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Dna Damage-Binding Protein 2
Regulatory Status
For Research Use Only
