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Human DHFR (Dihydrofolate Reductase) ELISA Kit
Human DHFR (Dihydrofolate Reductase) ELISA Kit
The Human (DHFR) Dihydrofolate Reductase ELISA Kit measures Dihydrofolate reductase in samples. The plate has been pre-coated with Human DHFR antibody. DHFR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DHFR Antibody is added and binds to DHFR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DHFR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DHFR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6651Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.092 ng/mL
Detection Range
0.2-70 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Cell Biology, Metabolism
Background
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Dihydrofolate reductase
Regulatory Status
For Research Use Only
