The Human (DHX32) Putative Pre-MRNA-Splicing Factor Atp-dependent Rna Helicase Dhx32 ELISA Kit measures Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 in samples. The plate has been pre-coated with Human DHX32 antibody. DHX32 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DHX32 Antibody is added and binds to DHX32 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DHX32 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DHX32. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPutative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 is an enzyme that in humans is encoded by the DHX32 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates two transcript variants, but the full length nature of one of the variants has not been defined. Source: UniProt Consortium (2025)
