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Human DIAPH1 (Protein Diaphanous Homolog 1) ELISA Kit
Human DIAPH1 (Protein Diaphanous Homolog 1) ELISA Kit
The Human (DIAPH1) Protein Diaphanous Homolog 1 ELISA Kit measures Protein Diaphanous Homolog 1 in samples. The plate has been pre-coated with Human DIAPH1 antibody. DIAPH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DIAPH1 Antibody is added and binds to DIAPH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DIAPH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DIAPH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2654Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.019 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed: 20937854, PubMed: 2183$498.00|$360.007, PubMed: 26912466). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed: 20937854, PubMed: 2183$498.00|$360.007). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed: 20937854, PubMed: 2183$498.00|$360.007). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed: 20937854, PubMed: 2183$498.00|$360.007). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed: 20937854, PubMed: 2183$498.00|$360.007). Plays a role in brain development (PubMed: 24781755). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Protein Diaphanous Homolog 1
Regulatory Status
For Research Use Only
