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Human DTX3L (E3 Ubiquitin-protein Ligase Dtx3L) ELISA Kit
Human DTX3L (E3 Ubiquitin-protein Ligase Dtx3L) ELISA Kit
The Human (DTX3L) E3 Ubiquitin-protein Ligase Dtx3L ELISA Kit measures E3 ubiquitin-protein ligase DTX3L in samples. The plate has been pre-coated with Human DTX3L antibody. DTX3L present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DTX3L Antibody is added and binds to DTX3L in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DTX3L antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DTX3L. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E7314Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.01 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Immunology
Background
E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed:12670957, PubMed:19818714, PubMed:26479788, PubMed:23230272).
Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed:28525742).
In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed:19818714).
The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed:19818714).
PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272).
By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed:26479788).
Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:24790097).
In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed:24790097).
In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation (PubMed:26479788). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
E3 ubiquitin-protein ligase DTX3L
Regulatory Status
For Research Use Only
