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Human EEF2 (Elongation Factor 2) ELISA Kit
Human EEF2 (Elongation Factor 2) ELISA Kit
The Human (EEF2) Elongation Factor 2 ELISA Kit measures elongation factor 2 in samples. The plate has been pre-coated with Human eEF2 antibody. eEF2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human eEF2 Antibody is added and binds to eEF2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated eEF2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human eEF2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5231Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.35 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
elongation factor 2
Regulatory Status
For Research Use Only
