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Human EEF2K (Eukaryotic Elongation Factor 2 Kinase) ELISA Kit
Human EEF2K (Eukaryotic Elongation Factor 2 Kinase) ELISA Kit
The Human (EEF2K) Eukaryotic Elongation Factor 2 Kinase ELISA Kit measures Eukaryotic Elongation Factor 2 Kinase in samples. The plate has been pre-coated with Human EEF2K antibody. EEF2K present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EEF2K Antibody is added and binds to EEF2K in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EEF2K antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human EEF2K. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6821Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.94 pg/mL
Detection Range
20-4500 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Enzyme & Kinase
Background
Threonine kinase that regulates protein synthesis by controlling the rate of Peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Eukaryotic Elongation Factor 2 Kinase
Regulatory Status
For Research Use Only
