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Human EGR2 (E3 Sumo-protein Ligase Egr2) ELISA Kit
Human EGR2 (E3 Sumo-protein Ligase Egr2) ELISA Kit
The Human (EGR2) E3 Sumo-protein Ligase Egr2 ELISA Kit measures E3 SUMO-protein ligase EGR2 in samples. The plate has been pre-coated with Human Egr2 antibody. Egr2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Egr2 Antibody is added and binds to Egr2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Egr2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human Egr2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3928Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.85 ng/L
Detection Range
10-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Immunology
Background
Sequence-specific DNA-binding transcription factor (PubMed:17717711).
Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (By similarity).
Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (By similarity).
Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (By similarity).
Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (By similarity).
Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).
Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (By similarity).
Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (By similarity).
May play a role in adipogenesis, possibly by regulating the expression of CEBPB (By similarity).
E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
E3 SUMO-protein ligase EGR2
Regulatory Status
For Research Use Only
