The Human (ENPP1) Ectonucleotide Pyrophosphatase, Phosphodiesterase Family Member 1 ELISA Kit measures Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 in samples. The plate has been pre-coated with Human ENPP1 antibody. ENPP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ENPP1 Antibody is added and binds to ENPP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ENPP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ENPP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundNucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (By similarity).
PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals (PubMed:11004006).
Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP (PubMed:27467858, PubMed:8001561, PubMed:25344812).
May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling (PubMed:27467858, PubMed:8001561).
Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (By similarity).
Appears to modulate insulin sensitivity and function (PubMed:10615944).
Also involved in melanogenesis (PubMed:28964717).
Also able to hydrolyze 2'-3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production (PubMed:25344812).
2'-3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2'-3'-cGAMP hydrolysis is therefore unclear (PubMed:25344812).
Not able to hydrolyze the 2'-3'-cGAMP linkage isomer 3'-3'-cGAMP (PubMed:25344812). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteEctonucleotide pyrophosphatase/phosphodiesterase family member 1
