The Human (ENPP3) Ectonucleotide Pyrophosphatase ELISA Kit measures Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 in samples. The plate has been pre-coated with Human ENPP3 antibody. ENPP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ENPP3 Antibody is added and binds to ENPP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ENPP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ENPP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundHydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed:29717535).
Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity).
Has also alkaline phosphodiesterase activity (PubMed:11342463). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteEctonucleotide pyrophosphatase/phosphodiesterase family member 3
