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Human ENPP5 ELISA Kit
Human ENPP5 ELISA Kit
This ELISA kit is designed to detect Human ENPP5. The assay plate has been pre-coated with mouse anti-Human ENPP-5 monoclonal antibody. When the sample containing ENPP-5 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human ENPP-5 Antibody is added to the wells and binds to ENPP-5 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human ENPP-5 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE199
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
12.3 pg/mL
Detection Range
46.88-3000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Neuroscience
Background
ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family (NPP). It is a family comprised of dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. However, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5
Regulatory Status
For Research Use Only
