The Human (ENTPD4) Ectonucleoside Triphosphate Diphosphohydrolase 4 ELISA Kit measures Ectonucleoside triphosphate diphosphohydrolase 4 in samples. The plate has been pre-coated with Human ENTPD4 antibody. ENTPD4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ENTPD4 Antibody is added and binds to ENTPD4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ENTPD4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ENTPD4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundCatalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP. AMP, ADP, ATP and UMP are not substrates (PubMed:10858452, PubMed:9556635).
Preferentially activated by Ca2+ over Mg2+ (PubMed:10858452).
Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP). Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP. Can use either Ca2+ or Mg2+ equally. Source: UniProt Consortium (2025)
