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Human EPHA1 (Ephrin Type-A Receptor 1) ELISA Kit

Human EPHA1 (Ephrin Type-A Receptor 1) ELISA Kit

The Human (EPHA1) Ephrin Type-A Receptor 1 ELISA Kit measures Ephrin Type-A Receptor 1 in samples. The plate has been pre-coated with Human EPHA1 antibody. EPHA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EPHA1 Antibody is added and binds to EPHA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EPHA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human EPHA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5244Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.031 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Ephrin Type-A Receptor 1
Regulatory Status For Research Use Only
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