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Human Ephrin Type-B Receptor 6 (EPHB6) ELISA Kit

Human Ephrin Type-B Receptor 6 (EPHB6) ELISA Kit

This ELISA kit is designed to detect Human Ephrin Type-B Receptor 6 (Human EPHB6). The assay plate has been pre-coated with mouse anti-Human EphB6 monoclonal antibody. When the sample containing EphB6 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human EphB6 Antibody is added to the wells and binds to EphB6 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human EphB6 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE041
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 2.82 pg/mL
Detection Range 9.38-600 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Neuroscience
Background Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Ephrin type-B receptor 6
Regulatory Status For Research Use Only
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