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Human EYA2 (Eyes Absent Homolog 2) ELISA Kit

Human EYA2 (Eyes Absent Homolog 2) ELISA Kit

The Human (EYA2) Eyes Absent Homolog 2 ELISA Kit measures Eyes Absent Homolog 2 in samples. The plate has been pre-coated with Human EYA2 antibody. EYA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human EYA2 Antibody is added and binds to EYA2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EYA2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human EYA2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5272Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 2.25 ng/L
Detection Range 5-1000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed: 12500905, PubMed: 23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed: 19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed: 12500905). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Eyes Absent Homolog 2
Regulatory Status For Research Use Only
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