The Human (FLRT3) Leucine-rich Repeat Transmembrane Protein Flrt3 ELISA Kit measures Leucine-rich repeat transmembrane protein FLRT3 in samples. The plate has been pre-coated with Human FLRT3 antibody. FLRT3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FLRT3 Antibody is added and binds to FLRT3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FLRT3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FLRT3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasSignal Transduction, Cancer, Developmental Biology
BackgroundFunctions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retina (By similarity).
Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:26235030).
Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (By similarity).
Promotes neurite outgrowth (in vitro) (PubMed:14706654).
Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length. Plays a role in the regulation of the density of glutamaergic synapses. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning. Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development. Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteLeucine-rich repeat transmembrane protein FLRT3
