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Human FN (Fibronectin) ELISA Kit
Human FN (Fibronectin) ELISA Kit
The Human (FN) Fibronectin ELISA Kit measures Fibronectin in samples. The plate has been pre-coated with Human FN1 antibody. FN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FN1 Antibody is added and binds to FN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2002Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
1.02 ng/mL
Detection Range
2-600 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed: 3024962, PubMed: 3900070, PubMed: 3593230, PubMed: 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed: 3024962, PubMed: 3900070, PubMed: 3593230, PubMed: 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Fibronectin
Regulatory Status
For Research Use Only
