The Human (FNDC3B) Fibronectin Type Iii Domain-containing Protein 3B ELISA Kit measures Fibronectin type III domain-containing protein 3B in samples. The plate has been pre-coated with Human FNDC3B antibody. FNDC3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FNDC3B Antibody is added and binds to FNDC3B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FNDC3B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FNDC3B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundThe Fibronectin type III domain is an evolutionary conserved protein domain that is widely found in animal proteins. The fibronectin protein in which this domain was first identified contains 16 copies of this domain. The domain is about 100 amino acids long and possesses a beta sandwich structure. Of the three fibronectin-type domains, type III is the only one without disulfide bonding present. Fibronectin domains are found in a wide variety of extracellular proteins. They are widely distributed in animal species, but also found sporadically in yeast, plant and bacterial proteins. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteFibronectin type III domain-containing protein 3B
