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Human FOXO3 (Forkhead Box O3) ELISA Kit
Human FOXO3 (Forkhead Box O3) ELISA Kit
The Human (FOXO3) Forkhead Box O3 ELISA Kit measures Forkhead Box O3 in samples. The plate has been pre-coated with Human FOXO3 antibody. FOXO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FOXO3 Antibody is added and binds to FOXO3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FOXO3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FOXO3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0626Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.33 ng/L
Detection Range
0.5-200 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed: 10102273, PubMed: 16751106, PubMed: 21329882). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed: 10102273, PubMed: 16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed: 21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed: 23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Forkhead Box O3
Regulatory Status
For Research Use Only
