The Human (GALNT11) Polypeptide N-acetylgalactosaminyltransferase 11 ELISA Kit measures Polypeptide N-Acetylgalactosaminyltransferase 11 in samples. The plate has been pre-coated with Human GALNT11 antibody. GALNT11 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GALNT11 Antibody is added and binds to GALNT11 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GALNT11 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GALNT11. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPolypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2 than GALNT1. Not involved in glycosylation of erythropoietin (EPO). Source: UniProt Consortium (2025)
