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Human GCH1 (Gtp Cyclohydrolase 1) ELISA Kit

Human GCH1 (Gtp Cyclohydrolase 1) ELISA Kit

The Human (GCH1) Gtp Cyclohydrolase 1 ELISA Kit measures Gtp Cyclohydrolase 1 in samples. The plate has been pre-coated with Human GCH1 antibody. GCH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GCH1 Antibody is added and binds to GCH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GCH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GCH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5366Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 7.47 ng/L
Detection Range 15-3000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Gtp Cyclohydrolase 1
Regulatory Status For Research Use Only
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