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Human GCLM (Glutamate--cysteine Ligase Regulatory Subunit) ELISA Kit
Human GCLM (Glutamate--cysteine Ligase Regulatory Subunit) ELISA Kit
The Human (GCLM) Glutamate--cysteine Ligase Regulatory Subunit ELISA Kit measures Glutamate--cysteine ligase regulatory subunit in samples. The plate has been pre-coated with Human GCLM antibody. GCLM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GCLM Antibody is added and binds to GCLM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GCLM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GCLM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5339Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
14.65 ng/L
Detection Range
30-9000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience, Immunology
Background
Glutamate-cysteine ligase regulatory subunit is an enzyme that in humans is encoded by the GCLM gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Glutamate--cysteine ligase regulatory subunit
Regulatory Status
For Research Use Only
