• Human (GSH) Glutathione ELISA Kit
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Human GSH (Glutathione) ELISA Kit

Human GSH (Glutathione) ELISA Kit

The Human (GSH) Glutathione ELISA Kit measures glutathione in samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of GSH in the sample. The concentration of GSH in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Catalog No: EA0142Hu
$595.00 USD
$458.00 USD $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.05 ng/mL
Detection Range 0.1 - 40 ng/mL
Sample Type Serum, plasma, cell culture supernatant
Incubation(s) 2.5 hour(s)
Research Areas Oxidative Stress
Background Glutathione (GSH) is an antioxidant in plants, animals, fungi, and some bacteria and archaea. Glutathione is capable of preventing damage to important cellular components caused by reactive oxygen species such as free radicals, peroxides, lipid peroxides, and heavy metals. It is a tripeptide with a gamma Peptide linkage between the carboxyl group of the glutamate side chain and cysteine. The carboxyl group of the cysteine residue is attached by normal Peptide linkage to glycine. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte glutathione
Regulatory Status For Research Use Only
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