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Human GSS (Glutathione Synthetase) ELISA Kit
Human GSS (Glutathione Synthetase) ELISA Kit
The Human (GSS) Glutathione Synthetase ELISA Kit measures Glutathione synthetase in samples. The plate has been pre-coated with Human GSS antibody. GSS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GSS Antibody is added and binds to GSS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GSS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GSS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5347Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.028 U/mL
Detection Range
0.05-20 U/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Neuroscience, Cell Biology, Metabolism, Oxidative Stress
Background
Glutathione synthetase (GSS) (EC 6.3.2.3) is the second enzyme in the glutathione (GSH) biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Glutathione synthetase is also a potent antioxidant. It is found in many species including bacteria, yeast, mammals, and plants. In humans, defects in GSS are inherited in an autosomal recessive way and are the cause of severe metabolic acidosis, 5-oxoprolinuria, increased rate of haemolysis, and defective function of the central nervous system. Deficiencies in GSS can cause a spectrum of deleterious symptoms in plants and human beings alike. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Glutathione synthetase
Regulatory Status
For Research Use Only
