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Human GTF2B (Transcription Initiation Factor IIB) ELISA Kit
Human GTF2B (Transcription Initiation Factor IIB) ELISA Kit
The Human (GTF2B) Transcription Initiation Factor IIB ELISA Kit measures Transcription initiation factor IIB in samples. The plate has been pre-coated with Human GTF2B antibody. GTF2B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GTF2B Antibody is added and binds to GTF2B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GTF2B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GTF2B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2709Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.77 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).
Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
Modulates transcription start site selection (PubMed:10318856).
Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Transcription initiation factor IIB
Regulatory Status
For Research Use Only
