The Human (GTF2H1) General Transcription Factor Iih Subunit 1 ELISA Kit measures General Transcription Factor Iih Subunit 1 in samples. The plate has been pre-coated with Human GTF2H1 antibody. GTF2H1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GTF2H1 Antibody is added and binds to GTF2H1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GTF2H1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GTF2H1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundComponent of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Source: UniProt Consortium (2025)
