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Human HAO1 (Hydroxyacidoxidase 1) ELISA Kit

Human HAO1 (Hydroxyacidoxidase 1) ELISA Kit

The Human (HAO1) Hydroxyacidoxidase 1 ELISA Kit measures Hydroxyacid Oxidase 1 in samples. The plate has been pre-coated with Human HAO1 antibody. HAO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HAO1 Antibody is added and binds to HAO1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HAO1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HAO1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5442Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 5.19 ng/L
Detection Range 10-2000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular
Background Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Hydroxyacid Oxidase 1
Regulatory Status For Research Use Only
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