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Human HEPH (Hephaestin) ELISA Kit
Human HEPH (Hephaestin) ELISA Kit
The Human (HEPH) Hephaestin ELISA Kit measures Hephaestin in samples. The plate has been pre-coated with Human HEPH antibody. HEPH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HEPH Antibody is added and binds to HEPH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HEPH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HEPH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2742Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.054 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Hephaestin, also known as HEPH, is a protein which in humans is encoded by the HEPH gene. Hephaestin is involved in the metabolism and homeostasis of iron and possibly copper.[8] It is a transmembrane copper-dependent ferroxidase responsible for transporting dietary iron from intestinal enterocytes into the circulatory system. The highest expression of hephaestin is found in small intestine. It is limited to enterocytes of the villi (where the iron absorption takes place), being almost absent in crypt cells. Hephaestin converts iron(II) state, Fe2+, to iron(III) state, Fe3+, and mediates iron efflux most likely in cooperation with the basolateral iron transporter, ferroportin 1. To a lesser extent hephaestin has been detected in colon, spleen, kidney, breast, placenta and bone trabecular cells but its role in these tissues remains to be established. Hephaestin presents homology with ceruloplasmin, a serum dehydrogenase protein involved in copper detoxification and storage. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Hephaestin
Regulatory Status
For Research Use Only
