The Human Herpud 1 ELISA Kit measures Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein in samples. The plate has been pre-coated with Human HERPUD1 antibody. HERPUD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HERPUD1 Antibody is added and binds to HERPUD1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HERPUD1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HERPUD1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundComponent of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:16289116, PubMed:28827405).
Could enhance presenilin-mediated amyloid-beta protein 40 generation. Binds to ubiquilins and this interaction is required for efficient degradation of CD3D via the ERAD pathway (PubMed:18307982). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteHomocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein
